Effective Practices for Description of Science Content within Digital Talking Books
Alternate Approach for Flow Chart
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In this alternate approach the description of the Large Flow Chart explicitly states when the different lines merge.
Large Flow Chart:
The large flow chart has three different starting points, presented here as separate lists with numbered steps. When a step has more than one possible next steps, they are listed beneath it.
Starting point A
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Funding for this project is provided by the National Science Foundation.
In this alternate approach the description of the Large Flow Chart explicitly states when the different lines merge.
Large Flow Chart:
The large flow chart has three different starting points, presented here as separate lists with numbered steps. When a step has more than one possible next steps, they are listed beneath it.
Starting point A
- Obtain AN12 strain
- Prepare genomic DNA
- Amplify ORF via PCR
- Ligate into pCR2,1-TOPO
- Ligate into pJP10
- Verify and sequence clone
- Transform into AN12
- Transform into AN12
- Verify and sequence clone
- Grow with and without IPTG
- Grow with and without IPTG
- Monitor carotenoid accumulation
- Obtain sequence of candidate genes
- Design primers to amplify target gene (ORF)
- Merges with Starting Point A, line 3: Amplify ORF via PCR
- Obtain maxiprep pJP10
- Merges with Starting Point A, line 5: Ligate into pJP10
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Funding for this project is provided by the National Science Foundation.